Mediator effect of luteolin on electrooxidation of NADH
نویسندگان
چکیده
The effects of luteolin (LU) as a new mediator on the electrooxidation reduced nicotinamide adenine dinucleotide (NADH) were investigated and developed disposable NADH sensor. Firstly, screen printed carbon electrodes (SPCE) modified with gold nanoparticles (AuNP), then, LU was deposited SPCE AuNP (SPCE/AuNP) using cyclic voltammetry. Electrochemical behavior SPCE/AuNP and, redox proses found to be adsorption controlled. number cycles optimized for electrochemical deposition determined 15 cycles. (SPCE/AuNP/LU) show electrocatalytic effect acts mediator. Working potential pH detection proposed SPCE/AuNP/LU sensor +0.225 V 7.25, respectively. showed well linear response from 15.90 925 ?M limit 4.57 sensitivity 11.19 ?AmM?1. repeatability tested 50 µM solution. relative standard deviation (RSD) calculated 3.68% (for n=10). operational stability studies have shown that initial amperometric decreased by 62.1% at 30th day. analysis in artificial human serum samples successfully performed
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ژورنال
عنوان ژورنال: Sakarya University Journal of Science
سال: 2021
ISSN: ['1301-4048', '2147-835X']
DOI: https://doi.org/10.16984/saufenbilder.745610